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dc.contributorSociedad Chilena de Anatomíaes_CL
dc.contributor.authorRojas, Robert [Chile. Universidad Mayor. Centro de Genómica y Bioinformática]es_CL
dc.contributor.authorSegovia, Christopher [Chile. Universidad Mayor. Centro de Genómica y Bioinformática]es_CL
dc.contributor.authorTrombert, Annette Nicole [Chile. Universidad Mayor. Centro de Genómica y Bioinformática]es_CL
dc.contributor.authorSantander, Javier [Chile. Universidad Mayor. Centro de Genómica y Bioinformática]es_CL
dc.contributor.authorManque, Patricio [Chile. Universidad Mayor. Centro de Genómica y Bioinformática]es_CL
dc.date.accessioned2018-08-23T00:21:07Z
dc.date.available2018-08-23T00:21:07Z
dc.date.issued2014es_CL
dc.identifier.citationRojas R, Segovia C, Trombert AN, Santander J, Manque P. The effect of tunicamycin on the glucose uptake, growth, and cellular adhesion in the protozoan parasite Crithidia fasciculata. Curr Microbiol. 2014 Oct;69(4):541-8. doi: 10.1007/s00284-014-0620-x. Epub 2014 Jun 4. PubMed PMID: 24894907.es_CL
dc.identifier.issnISSN 0717-9502es_CL
dc.identifier.urihttp://repositorio.umayor.cl/xmlui/handle/sibum/2601
dc.identifier.urihttps://link.springer.com/content/pdf/10.1007/s00284-014-0620-x.pdfes_CL
dc.description.abstractCrithidia fasciculata represents a very interesting model organism to study biochemical, cellular, and genetic processes unique to members of the family of the Trypanosomatidae. Thus, C. fasciculata parasitizes several species of insects and has been widely used to test new therapeutic strategies against parasitic infections. By using tunicamycin, a potent inhibitor of glycosylation in asparaginyl residues of glycoproteins (N-glycosylation), we demonstrate that N-glycosylation in C. fasciculata cells is involved in modulating glucose uptake, dramatically impacting growth, and cell adhesion. C. fasciculata treated with tunicamycin was severely affected in their ability to replicate and to adhere to polystyrene substrates and losing their ability to aggregate into small and large groups. Moreover, under tunicamycin treatment, the parasites were considerably shorter and rounder and displayed alterations in cytoplasmic vesicles formation. Furthermore, glucose uptake was significantly impaired in a tunicamycin dose-dependent manner; however, no cytotoxic effect was observed. Interestingly, this effect was reversible. Thus, when tunicamycin was removed from the culture media, the parasites recovered its growth rate, cell adhesion properties, and glucose uptake. Collectively, these results suggest that changes in the tunicamycin-dependent glycosylation levels can influence glucose uptake, cell growth, and adhesion in the protozoan parasite C. fasciculata.es_CL
dc.description.sponsorshipEste trabajo no declara proyecto(s) ni fondo(s) de financiamiento asociado(s)es_CL
dc.format.extentARTÍCULO ORIGINALes_CL
dc.language.isoenes_CL
dc.publisherFacultad de Cienciases_CL
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chilees_CL
dc.subjectCIENCIAS DE LA SALUDes_CL
dc.titleThe Effect of Tunicamycin on the Glucose Uptake, Growth, and Cellular Adhesion in the Protozoan Parasite Crithidia fasciculataes_CL
dc.typeArtículo o Paperes_CL
umayor.indizadorCOTes_CL
umayor.politicas.sherpa/romeoLicencia color: VERDE (Revista Scielo) --Licencia creative commons BYes_CL
umayor.indexadoSCOPUSes_CL
umayor.indexadoSCIELOes_CL
dc.identifier.doiEste artículo no posee número DOIes_CL]
umayor.indicadores.wos-(cuartil)Q3es_CL


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