| dc.contributor | Oxford University Press | es_CL |
| dc.contributor.author | Aguilera, Sergio [Chile. Universidad Mayor] | es_CL |
| dc.contributor.author | Olea, Nancy [Estados Unidos. NIH National Institute of Dental & Craniofacial Research] | es_CL |
| dc.contributor.author | González, Maria-Julieta [Universidad de Chile] | es_CL |
| dc.date.accessioned | 2018-09-07T13:04:11Z | |
| dc.date.available | 2018-09-07T13:04:11Z | |
| dc.date.issued | 2010 | es_CL |
| dc.identifier.citation | Pérez, P., Aguilera, S., Olea, N., Alliende, C., Molina, C., Brito, M., ... & González, M. J. (2010). Aberrant localization of ezrin correlates with salivary acini disorganization in Sjögren’s Syndrome. Rheumatology, 49(5), 915-923. | es_CL |
| dc.identifier.issn | ISSN 1462-0324 | es_CL |
| dc.identifier.issn | ESSN 1462-0332 | es_CL |
| dc.identifier.uri | https://academic.oup.com/rheumatology/article-pdf/49/5/915/17387032/keq033.pdf | es_CL |
| dc.identifier.uri | https://doi.org/10.1093/rheumatology/keq033 | es_CL |
| dc.identifier.uri | http://repositorio.umayor.cl/xmlui/handle/sibum/2628 | |
| dc.description.abstract | OBJECTIVES: To analyse whether the alterations in the structure and organization of microvilli in salivary acinar cells from SS patients are linked to changes in the expression and/or cellular localization of ezrin. METHODS: Salivary gland (SG) acini from controls and SS patients were used to evaluate ezrin expression by western blot and localization of total and activated (phospho-Thr567) ezrin by IF and EM. RESULTS: In acini from control labial SGs, ezrin was located predominantly at the apical pole and to a lesser extent at the basal region of these cells. Conversely, in acini extracts from SS patients, ezrin showed significantly elevated levels, which were accompanied with localization mostly at the basal region. Moreover, F-actin maintained its distribution in both the apical region and basolateral cortex; however, it was also observed in the acinar cytoplasm. Phospho-ezrin (active form) was located exclusively at the apical pole of acinar cells from control subjects and abundantly located at the basal cytoplasm in SS samples. These results were confirmed by immunogold studies. CONCLUSIONS: The decrease of ezrin and phospho-ezrin at the apical pole and the cytoplasmic redistribution of F-actin suggest an altered interaction between the F-actin-cytoskeleton and plasma membrane in SS patient acini, which may explain the microvilli disorganization. These alterations could eventually contribute to SG hyposecretion in SS patients. | es_CL |
| dc.description.sponsorship | Este trabajo fue financiado por: grants FONDECYT-CHILE 1080006, 1050192; PhD fellowships granted by Conicyt,Mecesup-Postgrade University of Chile 99-03. | es_CL |
| dc.format.extent | ARTÍCULO ORIGINAL | es_CL |
| dc.language.iso | en | es_CL |
| dc.publisher | CIENCIAS | es_CL |
| dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Chile | es_CL |
| dc.subject | REUMATOLOGÍA | es_CL |
| dc.title | Aberrant localization of ezrin correlates with salivary acini disorganization in Sjogren's Syndrome | es_CL |
| dc.type | Artículo o Paper | es_CL |
| umayor.indizador | COT | es_CL |
| umayor.politicas.sherpa/romeo | Licencia color: AMARILLO (Puede archivar el pre-print (ie la versión previa a la revisión por pares))--Pre-print del autor: el autor puede archivar la versión pre-print (ie la versión previa a la revisión por pares) Post-print del autor: el autor puede archivar la versión post-print (ie la versión final posterior a la revisión por pares) siempre que se cumplan las restricciones: 12 meses de embargo Versión de editor/PDF: cross el autor no puede archivar la versión del editor/PDF. Condiciones generales: El pre-print sólo puede depositarse antes de la aceptación, El pre-print debe acompañarse de una declaración establecida (ver enlace), El pre-print no debe reemplazarse por el post-print, sino que se enlazará a la versión publicada con una declaración establecida corregida, Pre-print on author's personal website, employer website, free public server or pre-prints in subject area, Post-print in Institutional repositories or Central repositories, La versión de editor/PDF no puede utilizarse, La fuente editorial debe reconocerse, Debe ir enlazado a la versión de editor, La copia archivada debe acompañarse de la frase establecida (ver Política), Eligible authors may deposit in OpenDepot, El editor depositará copia en PubMed Central en nombre de los autores financiados por el NIH// Disponible en: http://www.sherpa.ac.uk/romeo/issn/1462-0324/es/ | es_CL |
| umayor.indexado | WOS | es_CL |
| dc.identifier.doi | 10.1093/rheumatology/keq033 | es_CL] |
| umayor.indicadores.wos-(cuartil) | Q1 | es_CL |
| umayor.indicadores.scopus-(scimago-sjr) | sin información | es_CL |