| dc.contributor.author | del Pozo, Talia [Univ Mayor, Fac Ciencias, Ctr Conservac & Propagac Vegetal CEPROVEG, Santiago, Chile] | es_CL |
| dc.contributor.author | Bermudez, Luisa; Lira, Bruno Silvestre; de Godoy, Fabiana; Boos, Irene; Romano, Cecilia; Previtali, Viola; Almeida, Juliana; Brehelin, Claire; Asis, Ramon; Quadrana, Leandro; Demarco, Diego; Alseekh, Saleh; Salinas Gamboa, Rigel; Pérez-Flores, Laura; Guadalupe Dominguez, Pia; Rothan, Christophe; Fernie, Alisdair Robert; González, Mauricio; Stocker, Achim; Hemmerle, Andreas; Clausen, Mads Hartving; Carrari, Fernando; Rossi, Magdalena | es_CL |
| dc.date.accessioned | 2020-04-08T14:11:55Z | |
| dc.date.accessioned | 2020-04-13T18:12:46Z | |
| dc.date.available | 2020-04-08T14:11:55Z | |
| dc.date.available | 2020-04-13T18:12:46Z | |
| dc.date.issued | 2018 | es_CL |
| dc.identifier.citation | Bermudez Salazar, L., del Pozo, T., Lira, B. S., Godoy, F. D., Boos, I., Romano, C., ... & Quadrana, L. D. (2018). A tomato tocopherol-binding protein sheds light on intracellular a-tocopherol metabolism in plants. Oxford University Press. Plant and Cell Physiology, 59(11): 2188–2203, | es_CL |
| dc.identifier.issn | 0032-0781 | es_CL |
| dc.identifier.issn | 1471-9053 | es_CL |
| dc.identifier.uri | https://doi.org/10.1093/pcp/pcy191 | es_CL |
| dc.identifier.uri | http://repositorio.umayor.cl/xmlui/handle/sibum/6213 | |
| dc.description.abstract | Tocopherols are non-polar compounds synthesized in the plastids, which function as major antioxidants of the plant cells and are essential in the human diet. Both the intermediates and final products of the tocopherol biosynthetic pathway must cross plastid membranes to reach their sites of action. So far, no protein with tocopherol binding activity has been reported in plants. Here, we demonstrated that the tomato SlTBP protein is targeted to chloroplasts and able to bind alpha-tocopherol. SlTBP-knockdown tomato plants exhibited reduced levels of tocopherol in both leaves and fruits. Several tocopherol deficiency phenotypes were apparent in the transgenic lines, such as alterations in photosynthetic parameters, dramatic distortion of thylakoid membranes and significant variations in the lipid profile. These results, along with the altered expression of genes related to photosynthesis, and tetrapyrrole, lipid, isoprenoid, inositol/phosphoinositide and redox metabolism, suggest that SlTBP may act in conducting tocopherol (or its biosynthetic intermediates) between the plastid compartments and/or at the interface between chloroplast and endoplasmic reticulum membranes, affecting interorganellar lipid metabolism. | es_CL |
| dc.description.sponsorship | Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (Brazil; FAPESP)Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2010-50535-0]; Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq, Brazil)National Council for Scientific and Technological Development (CNPq); Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES, Brazil)CAPES; Universidade de Sao Paulo (USP, Brazil); Instituto Nacional de Tecnologia Agropecuaria (INTA, Argentina); Agencia Nacional de Promocion Cientifica y TecnologicaANPCyT; Facultad de Agronomia de la Universidad de Buenos Aires (FAUBA, Argentina); European Union Horizon 2020 Research and Innovation Programme [679796]; Fondo de Financiamiento de Centros de Investigacion em Areas Prioritarias (FONDAP, Chile) [50 15090007]; Fondo Nacional de Desarrollo Cientifico y Tecnologico (FONDECYT, Chile)Comision Nacional de Investigacion Cientifica y Tecnologica (CONICYT)CONICYT FONDECYT [11160899]; Novo Nordisk Foundation (Biotechnology-based Synthesis and Production Research); Villum Foundation (PLANET Project); Danish Agency for Science, Technology and Innovation (Danish-Brazilian Network for Plant Glycoscience); MINCYT-ECOS program 55 (Argentina-France); FAPESP fellowshipsFundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP); CNPqNational Council for Scientific and Technological Development (CNPq); Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET, Argentina)Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET) | es_CL |
| dc.description.sponsorship | This work was supported by Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (Brazil; FAPESP, 2010-50535-0); Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq, Brazil); Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES, Brazil); Universidade de Sao Paulo (USP, Brazil); Instituto Nacional de Tecnologia Agropecuaria (INTA, Argentina); Agencia Nacional de Promocion Cientifica y Tecnologica; Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET, Argentina); Facultad de Agronomia de la Universidad de Buenos Aires (FAUBA, Argentina); European Union Horizon 2020 Research and Innovation Programme (Grant Agreement Number 679796); Fondo de Financiamiento de Centros de Investigacion em Areas Prioritarias (FONDAP, 50 15090007, Chile); Fondo Nacional de Desarrollo Cientifico y Tecnologico (FONDECYT, 11160899, Chile); The Novo Nordisk Foundation (Biotechnology-based Synthesis and Production Research); The Villum Foundation (PLANET Project); the Danish Agency for Science, Technology and Innovation (Danish-Brazilian Network for Plant Glycoscience); and the MINCYT-ECOS program 55 (Argentina-France). L.B., B.S.L., F.G. and J.A. were recipients of FAPESP fellowships. L.B., M.R., and D.D. were funded by a fellowship from CNPq. L.B., R.A. and F.C. are members of the Argentine Council of Science and Technology (CONICET). | es_CL |
| dc.language.iso | en | es_CL |
| dc.publisher | OXFORD UNIV PRESS | es_CL |
| dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Chile | |
| dc.source | Plant Cell Physiol., NOV 2018. 59(11): p. 2188-2203 | |
| dc.subject | Plant Sciences; Cell Biology | es_CL |
| dc.title | A Tomato Tocopherol-Binding Protein Sheds Light on Intracellular alpha-Tocopherol Metabolism in Plants | es_CL |
| dc.type | Artículo | es_CL |
| umayor.facultad | CIENCIAS | es_CL |
| umayor.politicas.sherpa/romeo | Bronze, Green Published | es_CL |
| umayor.indexado | WOS:000457605600003 | es_CL |
| umayor.indexado | PMID: 30239816 | es_CL |
| dc.identifier.doi | DOI: 10.1093/pcp/pcy191 | es_CL] |
| umayor.indicadores.wos-(cuartil) | Q2 | es_CL |
| umayor.indicadores.scopus-(scimago-sjr) | SCIMAGO/ INDICE H: 135 H | es_CL |