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dc.contributor.authorSanhueza, Loreto; Muñoz, Patricia [Univ Mayor, Fac Estudios Interdisciplinarios, Nucleo Quim & Bioquim, Santiago, Chile]es_CL
dc.contributor.authorEspinoza, Javieres_CL
dc.contributor.authorUrzua, Alejandroes_CL
dc.contributor.authorWalter, Marianaes_CL
dc.contributor.authorFincheira, Paolaes_CL
dc.contributor.authorMuñoz, Patriciaes_CL
dc.contributor.authorMendoza, Leonoraes_CL
dc.contributor.authorWilkens, Marcelaes_CL
dc.date.accessioned2020-04-12T14:11:55Z
dc.date.accessioned2020-04-14T15:37:42Z
dc.date.available2020-04-12T14:11:55Z
dc.date.available2020-04-14T15:37:42Z
dc.date.issued2019es_CL
dc.identifier.citationEspinoza, J., Urzúa, A., Sanhueza, L., Walter, M., Fincheira, P., & Wilkens, M. (2019). Essential oil, extracts, and sesquiterpenes obtained from the heartwood of Pilgerodendron uviferum act as potential inhibitors of the Staphylococcus aureus NorA multidrug efflux pump. Frontiers in microbiology, 10, 337.es_CL
dc.identifier.issn1664-302Xes_CL
dc.identifier.urihttps://doi.org/10.3389/fmicb.2019.00337es_CL
dc.identifier.urihttp://repositorio.umayor.cl/xmlui/handle/sibum/6433
dc.description.abstractStaphylococcus aureus is a serious human pathogen that is highly adaptive to environmental conditions and rapidly develops antibiotic resistance. The use of efflux pumps to reduce antibiotic concentrations at the intracellular level is one of the main mechanisms by which bacteria develop antibiotic resistance. The management of efflux pumps, specifically NorA, which is expressed by S. aureus strains, is a valuable strategy for restoring susceptibility in strains resistant to antibacterial agents. In recent years, many studies have focused on searching for natural substances that can reverse efflux pump-mediated resistance in S. aureus. Extracts and compounds obtained from plants can be efficient efflux pump inhibitors (EPIs) and represent a potentially patient-friendly strategy for controlling S. aureus. In the present study, we evaluated the ability of essential oils, petroleum ether extracts, dichloromethane extract (DCME) and six compounds isolated from the heartwood of Pilgerodendron uviferum (Cupressaceae) and two synthetic derivatives to inhibit efflux in NorA pumps in the following three S. aureus strains: K2378, which overexpressed the norA gene (norA++), K1902 (norA-deleted, Delta norA) and the parental strain, NCTC 8325-4. Efflux activity was evaluated using a fluorometric method that measured the accumulation of the universal efflux pump substrate ethidium bromide (EtBr). Only DCME and the compounds 15-copaenol and epi-cubenol inhibited EtBr efflux by K2378. Even the lowest concentration of 15-copaenol exhibited a stronger inhibitory effect than carbonyl cyanide m-chlorophenyl hydrazone on EtBr efflux by K2378. 15-copaenal only showed inhibition of EtBr efflux in K2378 cells at 125 mu g/mL, but not superior to the control inhibitor and 15-copaenyl acetate exerted no intrinsic EPI activity against K2378. Fractional inhibitory concentration index (FICI) values obtained in the checkerboard assays, indicated that all combinations between DCME, epi-cubenol and 15-copaenol, and tested antibiotics showed a synergistic effect in wild type, norA and Delta norA strains. Moreover, those were not toxic for the HeLa cell line at concentrations in which the synergistic effect and inhibitory activity of efflux pumps was determined. Other extracts and compounds obtained from P. uviferum did not display EtBr efflux-inhibiting activity against the evaluated S. aureus strains.es_CL
dc.description.sponsorshipCONICYT-PCHA grant [2012-21120365]; CONICYT-Postdoctoral FONDECYT grants [3180532]; USACH Proyecto Basal USA [1799]es_CL
dc.description.sponsorshipThis research was funded by CONICYT-PCHA grant no. 2012-21120365, CONICYT-Postdoctoral FONDECYT grants no. 3180532 and USACH Proyecto Basal USA 1799.es_CL
dc.language.isoenes_CL
dc.publisherFRONTIERS MEDIA SAes_CL
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile
dc.sourceFront. Microbiol., FEB, 2019. 10
dc.subjectMicrobiologyes_CL
dc.titleEssential Oil, Extracts, and Sesquiterpenes Obtained From the Heartwood of Pilgerodendron uviferum Act as Potential Inhibitors of the Staphylococcus aureus NorA Multidrug Efflux Pumpes_CL
dc.typeArtículoes_CL
umayor.facultadCIENCIAS
umayor.politicas.sherpa/romeoDOAJ Gold, Green Publishedes_CL
umayor.indexadoWOS:000459662000001es_CL
umayor.indexadoPMID: 30863385es_CL
dc.identifier.doiDOI: 10.3389/fmicb.2019.00337es_CL]
umayor.indicadores.wos-(cuartil)Q1es_CL
umayor.indicadores.scopus-(scimago-sjr)SCIMAGO/ INDICE H: 88 Hes_CL


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