| dc.identifier.citation | Nuñez, C., Dupré, G., Mujica, K., Melet, L., Meisel, L., & Almeida, A. M. (2019). Thinning alters the expression of the PpeSUT1 and PpeSUT4 sugar transporter genes and the accumulation of translocated sugars in the fruits of an early season peach variety. Plant Growth Regulation, 88(3), 283-296. | es_CL |
| dc.description.abstract | The major human pathogens Trypanosoma cruzi, Trypanosoma brucei, and Leishmania major are collectively known as the Tritryps. The initial comparative analysis of their genomes has uncovered that Tritryps share a great number of genes, but repetitive DNA seems to be extremely variable between them. However, the in-depth characterization of repetitive DNA in these pathogens has been in part neglected, mainly due to the well-known technical challenges of studying repetitive sequences from de novo assemblies using short reads. Here, we compared the repetitive DNA repertories between the Tritryps genomes using genome-wide, low-coverage Illumina sequencing coupled to RepeatExplorer analysis. Our work demonstrates that this extensively implemented approach for studying higher eukaryote repeatomes is also useful for protozoan parasites like trypanosomatids, as we recovered previously observed differences in the presence and amount of repetitive DNA families. Additionally, our estimations of repetitive DNA abundance were comparable to those obtained from enhanced-quality assemblies using longer reads. Importantly, our methodology allowed us to describe a previously undescribed transposable element in Leishmania major (TATE element), highlighting its potential to accurately recover distinctive features from poorly characterized repeatomes. Together, our results support the application of this low-cost, low-coverage sequencing approach for the extensive characterization of repetitive DNA evolutionary dynamics in trypanosomatid and other protozoan genomes. | es_CL |
| dc.description.sponsorship | This work was funded by Agencia Nacional de Investigacion e Innovacion (UY) DCI-ALA/2011/023-502,"Contrato de apoyo a las politicas de innovacion y cohesion territorial', Fondo para la Convergencia Estructural del Mercado Comun del Sur (FOCEM) 03/11, and by Research Council United Kingdom Grand Challenges Research Funder 'A Global Network for Neglected Tropical Diseases' grant number MR/P027989/1. FDV has a ANII doctoral fellowship (No. POS_NAC_2016_1_129916). SP, GI and CR are members of the Sistema Nacional de Investigadores (SNI-ANII, UY).","OXFORD UNIV PRESS","1759-6653",,"Genome Biol. Evol., FEB, 2019. 11(2): p. 546-551","2019","DOI: 10.1093/gbe/evz017","https://doi.org/10.1093/gbe/evz017",,"Pita, S., Díaz-Viraqué, F., Iraola, G., & Robello, C. (2019). The Tritryps comparative repeatome: insights on repetitive element evolution in Trypanosomatid pathogens. Genome biology and evolution, 11(2), 546-551.","Evolutionary Biology; Genetics & Heredity",CIENCIAS,"WOS:000467953500017","PMID: 30715360",,"Q2","SCIMAGO/ INDICE H: 60 H","DOAJ Gold, Green Published","Attribution-NonCommercial-NoDerivs 3.0 Chile","2020-04-12T14:11:55Z","2020-04-12T14:11:55Z"
"+","sibum/65","Thinning alters the expression of the PpeSUT1 and PpeSUT4 sugar transporter genes and the accumulation of translocated sugars in the fruits of an early season peach variety",,"en","Artículo",,"Thinning is an agronomic practice that consists in the removal of a certain number of fruits, thereby altering the source-sink equilibrium in the tree and favoring carbon allocation to the remaining fruits. If thinning is performed at the appropriate fruit developmental stage and at an adequate intensity, the remaining fruits will have a larger final diameter and higher soluble solids content. However, the amount of carbon translocated to a fruit depends on the photosynthetic rate in the source leaves, the production of translocation sugars, as well as the fruit sink strength, which is directly related to the phloem unloading. It is not clear which of these steps is altered by thinning. In this work, we evaluated the effect of thinning in an early season peach variety. Photosynthetic parameters, quantification of soluble sugars in leaves and fruits, and the expression of sucrose and sorbitol transporters were evaluated in leaves and fruits during whole fruit development. We showed that thinning was effective in improving final fruit size and sugar accumulation without inhibiting photosynthesis. We also observed a 10-day delay in stone lignification when the trees were not thinned. The stone lignification phase was very short in the thinned treatment, while it seems to last for longer under unthinning. Furthermore, it was shown that thinning affects the expression of translocation sugar transporters genes (PpeSUT1 and PpeSUT4) in both leaves and fruits, which could be increasing the fruit sink strength and subsequently improving translocation sugars transport to the fruit when trees are thinned.","Miyasaka Almeida, Andrea [Univ Mayor, Ctr Genom & Bioinformat, Fac Ciencias]","Núñez, Carlos [Univ Mayor, Ctr Genom & Bioinformat, Fac Ciencias]","Dupre, German","Mujica, Karen","Melet, Lorena","Meisel, Lee",,,,,"Fondo Nacional de Desarrollo Cientifico y TecnologicoComision Nacional de Investigacion Cientifica y Tecnologica (CONICYT)CONICYT FONDECYT [Fondecyt 1130197, Fondecyt 1171016]; [CONICYT-PCHA/Doctorado National/2014-21140426]","This research was supported by Fondo Nacional de Desarrollo Cientifico y Tecnologico, Fondecyt 1130197 to AMA and Fondecyt 1171016 to LM. KM is a PhD student supported by CONICYT-PCHA/Doctorado National/2014-21140426. We thank El Tambo nursery to give us access to their fields to realize the experiments. We also thank Dr Denise Rougier for the assistance with histology analysisand Camilo Avedano for the assistance on"Sweet September' fruit measurements in the field. | es_CL |