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dc.contributor.authorPavez B., Saavedra A., Gentina J.C.es_CL
dc.contributor.authorDíaz, Mauricio [Institute of Biotechnology, Universidad Mayor, Chile]es_CL
dc.date.accessioned2020-08-12T14:11:55Z
dc.date.accessioned2020-08-12T19:30:40Z
dc.date.available2020-08-12T14:11:55Z
dc.date.available2020-08-12T19:30:40Z
dc.date.issued2013es_CL
dc.identifier.citationPavez, B., Saavedra, A., Diaz, M., & Gentina, J. C. (2013). Effect of Exogenous Galactose on EPS Production during Bioleaching of Pyrite by Acidithiobacillus ferrooxidans. Advanced Materials Research, 825, 125–128. https://doi.org/10.4028/www.scientific.net/amr.825.125es_CL
dc.identifier.issn1022-6680es_CL
dc.identifier.urihttps://www.scientific.net/AMR.825.125es_CL
dc.identifier.urihttps://doi.org/10.4028/www.scientific.net/AMR.825.125es_CL
dc.identifier.urihttp://repositorio.umayor.cl/xmlui/handle/sibum/6981
dc.description.abstractExtracellular polymeric substances (EPS) play an important role in the attachment of bacteria to sulphide minerals, biofilm formation and efficiency of the bioleaching process. Previous studies have suggested a potential connection between galactose and EPS formation. In this context, the influence of exogenous galactose on EPS formation during the bioleaching of pyrite was studied. In order to fully adapt the microorganism to bioleaching conditions it was performed a total of five consecutive sub cultures, one every fifteen days, taking for each one inocula from previous culture in shake flasks with 200 ml of fermentation medium at 30°C, 200 rpm, 40 gL-1 mineral and an initial pH of 1,8. Assays were performed in a medium supplemented with exogenous galactose (0.25% w/v) and without exogenous galactose (control), both with an initial concentration of ferric sulphate in the first three sub cultures of 5 gL-1, decreasing in the last two sub cultures to 2.5 gL-1. Samples of three cultures in both conditions were analyzed using confocal laser scanning microscopy (CLSM) labelling the cells with propidium iodide and EPS carbohydrates with Wheat Germ Agglutinin (WGA). Samples obtained on the last day of the fifth culture showed that the EPS layer on the particle surface was 5.00 μm3/μm2 in the case of the control condition and 6.10 μm3/μm2 when bioleaching was carried out in the presence of exogenous galactose. Also it was observed that in the fifth sub culture the volumetric productivity of total iron in the control experiment was 0.0065 gL-1.h-1 compared with 0.0076 gL-1.h-1 obtained in presence of galactose. The results reveal that the presence of galactose in the bioleaching solution stimulates EPS's formation and apparently also favour the pyrite bioleaching process.es_CL
dc.format.extentArtículo original
dc.language.isoenes_CL
dc.publisherTrans Tech Publicationses_CL
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile
dc.sourceAdvanced Materials Research, 2013. 825(): p: 125-128
dc.titleEffect of exogenous galactose on EPS production during bioleaching of pyrite by Acidithiobacillus ferrooxidanses_CL
dc.typeArtículo o paperes_CL
umayor.facultadFacultad de Ciencias
umayor.indizadorCOT
umayor.politicas.sherpa/romeoSuscripciónes_CL
umayor.indexadoSCOPUSes_CL
dc.identifier.doiDOI: 10.4028/www.scientific.net/amr.825.125es_CL]
umayor.indicadores.scopus-(scimago-sjr)0es_CL
umayor.indicadores.scopus-(scimago-sjr)ÍNDICE H: 37es_CL


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