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| dc.contributor | Springer Nature | es |
| dc.contributor.author | Contreras, Fernanda [Centro de Genómica y Bioinformática, Facultad de Ciencias, Universidad Mayor, Chile] | |
| dc.contributor.author | Rivas-Pardo, Jaime Andrés [Centro de Genómica y Bioinformática, Facultad de Ciencias, Universidad Mayor, Chile] | |
| dc.date.accessioned | 2020-12-17T19:38:14Z | |
| dc.date.available | 2020-12-17T19:38:14Z | |
| dc.date.issued | 2020-05-20 | |
| dc.identifier.citation | Contreras F., Rivas-Pardo J.A. (2020) Interfering with the Folding of Group A Streptococcal pili Proteins. In: Proft T., Loh J. (eds) Group A Streptococcus. Methods in Molecular Biology, vol 2136. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0467-0_28 | es |
| dc.identifier.isbn | 9781071604663 | |
| dc.identifier.isbn | 9781071604670 | |
| dc.identifier.uri | http://repositorio.umayor.cl/xmlui/handle/sibum/7266 | |
| dc.identifier.uri | https://doi.org/10.1007/978-1-0716-0467-0_28 | |
| dc.identifier.uri | https://link.springer.com/protocol/10.1007/978-1-0716-0467-0_28#citeas | |
| dc.identifier.uri | https://cgb.umayor.cl/publicaciones/interfering-with-the-folding-of-group-a-streptococcal-pili-proteins | |
| dc.description.abstract | Gram-positive bacteria use their adhesive pili to attach to host cells during early stages of a bacterial infection. These extracellular hair-like appendages experience mechanical stresses of hundreds of picoNewtons; however, the presence of an internal isopeptide bond prevents the pilus protein from unfolding. Here, we describe a method to interfere with nascent pili proteins through a peptide that mimics one of the β-strands of the molecule. By using AFM-based force spectroscopy, we study the isopeptide bond formation and the effect of the peptide in the elasticity of the pilus protein. This method could be used to afford a new strategy for mechanically targeted antibiotics by simply blocking the folding of the bacterial pilus protein. | es |
| dc.description.sponsorship | This work was supported by FONDECYT 11180705 (J.A.R-P.). We thank all the members of the Rivas-Pardo’s lab and the Inmunogenomics and Applied Genomics Laboratories for their helpful discussions. | es |
| dc.format.extent | 395 p., PDF | es |
| dc.language.iso | en | es |
| dc.publisher | Chile. Universidad Mayor | es |
| dc.rights | Copyright | es |
| dc.title | Interfering with the Folding of Group A Streptococcal pili Proteins | es |
| dc.type | Artículo o Paper | es |
| umayor.indizador | COT | es |
| umayor.politicas.sherpa/romeo | Esta obra está protegida bajo licencia de copyright | es |
| umayor.indexado | Scopus | es |
| dc.identifier.doi | 10.1007/978-1-0716-0467-0_28 | |
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