Taxonomic reassignment of Pseudohaptolina birgeri comb. nov. (Haptophyta)

Abstract

The haptophyte genus Pseudohaptolina (formerly Chrysochromulina clade B1-3) currently harbours two species: Pseudohaptolina arctica and Pseudohaptolina sorokinii. In addition, Chrysochromulina birgeri is expected to belong to this genus due to its morphological similarity to P. sorokinii, but it has not yet been genetically characterised. A strain belonging to Pseudohaptolina was brought into culture from Arctic waters, characterised by 18S and 28S rRNA gene sequencing as well as optical and transmission electron microscopy, and deposited in the Roscoff Culture Collection with the code RCC5270. Molecular and morphological data from RCC5270 were compared with those from previously described Pseudohaptolina and Pseudohaptolina-like species. Strain RCC5270 showed strong phylogenetic affinity to P. sorokinii, but observations using transmission electron microscopy (TEM) showed that RCC5270 possesses three types of organic body scales, rather than two as originally described for P. sorokinii. We found that the occurrence of three scale types is likely to have been overlooked in the original descriptions of both P. sorokinii and C. birgeri. We also found that environmental metabarcodes identical to the sequence of RCC5270 were abundant at the location from which C. birgeri was initially described (Gulf of Finland). We conclude that P. sorokinii and C. birgeri are conspecific and P. sorokinii is therefore synonymous with C. birgeri. Based on its phylogenetic placement and nomenclatural priority, we propose the new combination Pseudohaptolina birgeri and emend the description of this species.

The haptophyte genus Pseudohaptolina (formerly Chrysochromulina clade B1-3) currently harbours two species: Pseudohaptolina arctica and Pseudohaptolina sorokinii. In addition, Chrysochromulina birgeri is expected to belong to this genus due to its morphological similarity to P. sorokinii, but it has not yet been genetically characterised. A strain belonging to Pseudohaptolina was brought into culture from Arctic waters, characterised by 18S and 28S rRNA gene sequencing as well as optical and transmission electron microscopy, and deposited in the Roscoff Culture Collection with the code RCC5270. Molecular and morphological data from RCC5270 were compared with those from previously described Pseudohaptolina and Pseudohaptolina-like species. Strain RCC5270 showed strong phylogenetic affinity to P. sorokinii, but observations using transmission electron microscopy (TEM) showed that RCC5270 possesses three types of organic body scales, rather than two as originally described for P. sorokinii. We found that the occurrence of three scale types is likely to have been overlooked in the original descriptions of both P. sorokinii and C. birgeri. We also found that environmental metabarcodes identical to the sequence of RCC5270 were abundant at the location from which C. birgeri was initially described (Gulf of Finland). We conclude that P. sorokinii and C. birgeri are conspecific and P. sorokinii is therefore synonymous with C. birgeri. Based on its phylogenetic placement and nomenclatural priority, we propose the new combination Pseudohaptolina birgeri and emend the description of this species.