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dc.contributorElsevieres_CL
dc.contributor.authorBarrera, María José [Universidad de Chile]es_CL
dc.contributor.authorSánchez, Marianela [Universidad de Chile]es_CL
dc.contributor.authorAguilera, Sergio [Chile. Clínica INDISA]es_CL
dc.contributor.authorAlliende, Cecilia [Universidad de Chile]es_CL
dc.contributor.authorBahamondes, Verónica [Universidad de Chile]es_CL
dc.contributor.authorMolina, Claudio [Chile. Universidad Mayor]es_CL
dc.contributor.authorLeyton, Cecilia [Universidad de Chile]es_CL
dc.contributor.authorQuest, Andrew F.G. [Universidad de Chile]es_CL
dc.contributor.authorUrzúa, Ulises [Universidad de Chile]es_CL
dc.contributor.authorCastro, Isabel [Universidad de Chile]es_CL
dc.contributor.authorGonzález, Sergio [Chile. Universidad Mayor]es_CL
dc.contributor.authorSung, Hsiao Hsin [Universidad de Chile]es_CL
dc.contributor.authorAlbornoz, Amelina [Universidad de Chile]es_CL
dc.contributor.authorHermoso, Marcela [Universidad de Chile]es_CL
dc.contributor.authorGonzález, María-Julieta [Universidad de Chile]es_CL
dc.date.accessioned2018-08-23T00:20:57Z
dc.date.available2018-08-23T00:20:57Z
dc.date.issued2012es_CL
dc.identifier.citationBarrera MJ, Sánchez M, Aguilera S, Alliende C, Bahamondes V, Molina C, Quest AF, Urzúa U, Castro I, González S, Sung HH, Albornoz A, Hermoso M, Leyton C, González MJ. Aberrant localization of fusion receptors involved in regulated exocytosis in salivary glands of Sjögren's syndrome patients is linked to ectopic mucin secretion. J Autoimmun. 2012 Aug;39(1-2):83-92. doi: 10.1016/j.jaut.2012.01.011. Epub 2012 Jan 30. PubMed PMID: 22285554.es_CL
dc.identifier.issnISSN: 0896-8411es_CL
dc.identifier.urihttp://repositorio.umayor.cl/xmlui/handle/sibum/2490
dc.identifier.urihttps://ac.els-cdn.com/S0896841112000121/1-s2.0-S0896841112000121-main.pdf?_tid=87b74692-7ac7-42d7-bc7e-d3e41d976ab0&acdnat=1534445769_89dace03896db4bdaa4789c544388c0fes_CL
dc.identifier.urihttps://doi.org/10.1016/j.jaut.2012.01.011es_CL
dc.description.abstractSjögren's syndrome (SS) is a chronic inflammatory autoimmune disease that mainly affects tear and salivary glands, whereby SS-patients frequently complain of eye and mouth dryness. Salivary acinar cells of SS-patients display alterations in their cell polarity; which may affect the correct localization and function of proteins involved in regulated exocytosis. Here we determined whether the expression and localization of SNARE proteins (membrane fusion receptors) involved in regulated secretion, such as VAMP8, syntaxin 3 (STX3), STX4 and SNAP-23 were altered in salivary glands (SG) from SS-patients. Additionally, we investigated SNARE proteins function, by evaluating their ability to form SNARE complexes under basal conditions. In SG from SS-patients and control subjects mRNA and proteins levels of SNARE complex components were determined by real-time PCR and Western blotting, respectively. SNARE protein distribution and mucin exocytosis were determined by indirect immunofluorescence. In SS-patients, the expression levels of mRNA and protein for VAMP8, STX4 and STX3 were altered. STX4, STX3, SNAP-23 and VAMP8 relocated from the apical to the basal region of acinar cells. Increased formation of SNARE complexes in a manner independent of external stimuli for secretion was detected. Mucins were detected in the extracellular matrix (ECM). Presence of mucins in the ECM, together with the observed alterations in SNARE protein localization is indicative of ectopic exocytosis. In the context of SS, such aberrantly localized mucins are likely to favor a pro-inflammatory response, which may represent an important initial step in the pathogenesis of this disease.es_CL
dc.description.sponsorshipEste trabajo fue financiado por proyecto Fondecyt-Chile 1080006, (MJG, SA, CM), PhD fellowship Conicyt-Chile (MJB), FONDAP 15010006 (AFGQ).es_CL
dc.format.extentARTÍCULO ORIGINALes_CL
dc.language.isoenes_CL
dc.publisherFacultad de Cienciases_CL
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chilees_CL
dc.subjectCIENCIAS DE LA SALUDes_CL
dc.titleAberrant localization of fusion receptors involved in regulated exocytosis in salivary glands of Sjogren's syndrome patients is linked to ectopic mucin secretion.es_CL
dc.typeArtículo o Paperes_CL
umayor.indizadorCOTes_CL
umayor.politicas.sherpa/romeoLicencia color: VERDE C/RSe puede archivar el pre-print y el post-print o versión de editor/PDF, el autor no puede archivar la versión del editor/PDF) --Authors pre-print on any website, including arXiv and RePEC Author's post-print on author's personal website immediately Author's post-print on open access repository after an embargo period of between 12 months and 48 months Permitted deposit due to Funding Body, Institutional and Governmental policy or mandate, may be required to comply with embargo periods of 12 months to 48 months Author's post-print may be used to update arXiv and RepEC La versión de editor/PDF no puede utilizarse Debe enlazar a la versión de editor con DOI Author's post-print must be released with a Creative Commons Attribution Non-Commercial No Derivatives Licensees_CL
umayor.indexadoSCOPUSes_CL
dc.identifier.doi10.1016/j.jaut.2012.01.011es_CL]


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