| dc.contributor.author | Orell, Alvaro [Univ Mayor, Fac Ciencias, Ctr Genom & Bioinformat, Santiago, Chile] | es_CL |
| dc.contributor.author | Maracaja-Coutinho, Vinicius [Univ Mayor, Fac Ciencias, Ctr Genom & Bioinformat, Santiago, Chile] | es_CL |
| dc.contributor.author | Aliaga-Tobar, Victor [Univ Mayor, Vicerrectoria Invest, Programa Doctorado Genom Integrat, Santiago, Chile] | es_CL |
| dc.contributor.author | Tripp, Vanessa | es_CL |
| dc.contributor.author | Albers, Sonja-Verena | es_CL |
| dc.contributor.author | Randau, Lennart | es_CL |
| dc.date.accessioned | 2020-04-08T14:11:55Z | |
| dc.date.accessioned | 2020-04-13T18:12:35Z | |
| dc.date.available | 2020-04-08T14:11:55Z | |
| dc.date.available | 2020-04-13T18:12:35Z | |
| dc.date.issued | 2018 | es_CL |
| dc.identifier.citation | Orell, A., Tripp, V., Aliaga-Tobar, V., Albers, S. V., Maracaja-Coutinho, V., & Randau, L. (2018). A regulatory RNA is involved in RNA duplex formation and biofilm regulation in Sulfolobus acidocaldarius. Nucleic acids research, 46(9), 4794-4806. | es_CL |
| dc.identifier.issn | 0305-1048 | es_CL |
| dc.identifier.issn | 1362-4962 | es_CL |
| dc.identifier.uri | https://doi.org/10.1093/nar/gky144 | es_CL |
| dc.identifier.uri | http://repositorio.umayor.cl/xmlui/handle/sibum/6105 | |
| dc.description.abstract | Non-coding RNAs (ncRNA) are involved in essential biological processes in all three domains of life. The regulatory potential of ncRNAs in Archaea is, however, not fully explored. In this study, RNA-seq analyses identified a set of 29 ncRNA transcripts in the hyperthermophilic archaeon Sulfolobus acidocaldarius that were differentially expressed in response to biofilm formation. The most abundant ncRNA of this set was found to be resistant to RNase R treatment (RNase R resistant RNA, RrrR(+)) due to duplex formation with a reverse complementary RNA (RrrR(-)). The deletion of the RrrR(+) gene resulted in significantly impaired biofilm formation, while its overproduction increased biofilm yield. RrrR(+) was found to act as an antisense RNA against the mRNA of a hypothetical membrane protein. The RrrR(+) transcript was shown to be stabilized by the presence of the RrrR(-) strand in S. acidocaldarius cell extracts. The accumulation of these RrrR duplexes correlates with an apparent absence of dsRNA degrading RNase III domains in archaeal proteins. | es_CL |
| dc.description.sponsorship | Deutsche ForschungsgemeinschaftGerman Research Foundation (DFG) [DFG RA 2169/3-1]; Comision Nacional de Ciencia y Tecnologia de Chile [FONDECYT 1130229]; Max Planck SocietyMax Planck Society | es_CL |
| dc.description.sponsorship | Deutsche Forschungsgemeinschaft [DFG RA 2169/3-1 to L.R.]; Comision Nacional de Ciencia y Tecnologia de Chile [FONDECYT 1130229 to A.O]. Funding for open access charge: Max Planck Society. | es_CL |
| dc.language.iso | en | es_CL |
| dc.publisher | OXFORD UNIV PRESS | es_CL |
| dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Chile | |
| dc.source | Nucleic Acids Res., MAY 2018. 46(9): p. 4794-4806 | |
| dc.subject | Biochemistry & Molecular Biology | es_CL |
| dc.title | A regulatory RNA is involved in RNA duplex formation and biofilm regulation in Sulfolobus acidocaldarius | es_CL |
| dc.type | Artículo | es_CL |
| umayor.facultad | CIENCIAS | es_CL |
| umayor.politicas.sherpa/romeo | DOAJ Gold, Green Published | es_CL |
| umayor.indexado | WOS:000433056900040 | es_CL |
| umayor.indexado | PMID: 29529252 | es_CL |
| dc.identifier.doi | DOI: 10.1093/nar/gky144 | es_CL] |
| umayor.indicadores.wos-(cuartil) | Q1 | es_CL |
| umayor.indicadores.scopus-(scimago-sjr) | SCIMAGO/ INDICE H: 452 H | es_CL |