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dc.contributor.authorSepúlveda-Cisternas, Ignacio [Univ Mayor, Escuela Biotecnol, Campus Huechuraba, Santiago, Chile]es_CL
dc.contributor.authorLozano Aguirre, Luises_CL
dc.contributor.authorFuentes Flores, Andréses_CL
dc.contributor.authorSolis de Ovando, Ignacio Vásquezes_CL
dc.contributor.authorAntonio García-Angulo, Victores_CL
dc.date.accessioned2020-04-08T14:11:55Z
dc.date.accessioned2020-04-13T18:12:55Z
dc.date.available2020-04-08T14:11:55Z
dc.date.available2020-04-13T18:12:55Z
dc.date.issued2018es_CL
dc.identifier.citationSepúlveda-Cisternas, I., Aguirre, L. L., Flores, A. F., de Ovando, I. V. S., & García-Angulo, V. A. (2018). Transcriptomics reveals a cross-modulatory effect between riboflavin and iron and outlines responses to riboflavin biosynthesis and uptake in Vibrio cholerae. Scientific reports, 8(1), 1-14.es_CL
dc.identifier.issn2045-2322es_CL
dc.identifier.urihttps://doi.org/10.1038/s41598-018-21302-3es_CL
dc.identifier.urihttp://repositorio.umayor.cl/xmlui/handle/sibum/6308
dc.description.abstractVibrio cholerae, a pandemic diarrheagenic bacterium, is able to synthesize the essential vitamin riboflavin through the riboflavin biosynthetic pathway (RBP) and also to internalize it through the RibN importer. In bacteria, the way riboflavin biosynthesis and uptake functions correlate is unclear. To gain insights into the role of the riboflavin provision pathways in the physiology of V. cholerae, we analyzed the transcriptomics response to extracellular riboflavin and to deletions of ribD (RBP-deficient strain) or ribN. Many riboflavin-responsive genes were previously reported to belong to the iron regulon, including various iron uptake genes. Real time PCR analysis confirmed this effect and further documented that reciprocally, iron regulates RBP and ribN genes in a riboflavin-dependent way. A subset of genes were responding to both ribD and ribN deletions. However, in the subset of genes specifically affected in the Delta ribD strain, the functional terms protein folding and oxidation reduction process were enriched, as determined by a Gene Ontology analysis. In the gene subset specifically affected in the Delta ribN strain, the cytochrome complex assembly functional term was enriched. Results suggest that iron and riboflavin interrelate to regulate its respective provision genes and that both common and specific effects of biosynthesized and internalized riboflavin exist.es_CL
dc.description.sponsorshipCONICYT-FONDECYT (Chile)Comision Nacional de Investigacion Cientifica y Tecnologica (CONICYT)CONICYT FONDECYT [1150818]es_CL
dc.description.sponsorshipWe thank Daniela Gutiérrez for logistic support for transcriptomics analysis. This work was funded by CONICYT-FONDECYT (Chile) Grant Number 1150818.es_CL
dc.language.isoenes_CL
dc.publisherNATURE PUBLISHING GROUPes_CL
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile
dc.sourceSci Rep, FEB 2018. 8
dc.subjectMultidisciplinary Scienceses_CL
dc.titleTranscriptomics reveals a cross-modulatory effect between riboflavin and iron and outlines responses to riboflavin biosynthesis and uptake in Vibrio choleraees_CL
dc.typeArtículoes_CL
umayor.facultadCIENCIASes_CL
umayor.politicas.sherpa/romeoDOAJ Gold, Green Publishedes_CL
umayor.indexadoWOS:000425284900017es_CL
umayor.indexadoSIN PMIDes_CL
dc.identifier.doiDOI: 10.1038/s41598-018-21302-3es_CL]
umayor.indicadores.wos-(cuartil)Q1es_CL
umayor.indicadores.scopus-(scimago-sjr)SCIMAGO/ INDICE H: 149 Hes_CL


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