Vista simple de metadatos

dc.contributor.authorIraola, Gregorio [Univ Mayor, Ctr Integrat Biol, Santiago, Chile]es_CL
dc.contributor.authorRobello, Carlos; Patricia Maldonado, Doris; Hevia, Anna; Hoashi, Marina; Frattaroli, Paola; Montacutti, Valentina; Heguy, Adriana; Dolgalev, Igor; Mojica, Maricruz; Dominguez-Bello, María G.es_CL
dc.date.accessioned2020-04-12T14:11:55Z
dc.date.accessioned2020-04-14T15:37:42Z
dc.date.available2020-04-12T14:11:55Z
dc.date.available2020-04-14T15:37:42Z
dc.date.issued2019es_CL
dc.identifier.citationRobello, C., Maldonado, D. P., Hevia, A., Hoashi, M., Frattaroli, P., Montacutti, V., ... & Dominguez-Bello, M. G. (2019). The fecal, oral, and skin microbiota of children with Chagas disease treated with benznidazole. PloS one, 14(2).es_CL
dc.identifier.issn1932-6203es_CL
dc.identifier.urihttps://doi.org/10.1371/journal.pone.0212593es_CL
dc.identifier.urihttp://repositorio.umayor.cl/xmlui/handle/sibum/6432
dc.description.abstractBackground Chagas disease is still prevalent in rural areas of South America. In endemic areas of Bolivia, school children are screened for the program of Chagas disease eradication of the Ministry of Health, and positive children are treated. Here, we compared the fecal, oral and skin microbiomes of children with or without Chagas disease, and before and after benznidazol treatment of infected children. Methods A total of 543 Bolivian children (5-14 years old) were tested for Chagas disease, and 20 positive children were treated with Benznidazole. Fecal samples and oral and skin swabs were obtained before and after treatment, together with samples from a group of 35 uninfected controls. The 16S rRNA genes were sequenced and analyzed using QIIME to determine Alpha diversity differences and community distances, and linear discriminant analyses to determine marker taxa by infection status or treatment. Results Twenty out of 543 children screened were seropositive for Chagas disease (3.7%) and were included in the study, together with 35 control children that were seronegative for the disease. Fecal samples, oral and skin swabs were taken at the beginning of the study and after the anti-protozoa therapy with Benznidazole to the chagasic children. Infected children had higher fecal Firmicutes (Streptococcus, Roseburia, Butyrivibrio, and Blautia), and lower Bacteroides and also showed some skin -but not oral-microbiota differences. Treatment eliminated the fecal microbiota differences from control children, increasing Dialister (class Clostridia) and members of the Enterobacteriaceae, and decreasing Prevotella and Coprococcus, with minor effects on the oral and skin bacterial diversity. Conclusions The results of this study show differences in the fecal microbiota associated with Chagas disease in children, and also evidence that treatment normalizes fecal microbiota (makes it more similar to that in controls), but is associated with oral and skin microbiota differences from control children. Since microbiota impacts in children, it is important to determine the effect of drugs on the children microbiota, since dysbiosis could lead to physiological effects which might be avoidable with microbiota restoration interventions.es_CL
dc.description.sponsorshipC&D Fund for Microbial Anthropology; Emch Fundes_CL
dc.description.sponsorshipWe acknowledge the support of the C&D Fund for Microbial Anthropology, and the Emch Fund. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.es_CL
dc.language.isoenes_CL
dc.publisherPUBLIC LIBRARY SCIENCEes_CL
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile
dc.sourcePLoS One, FEB, 2019. 14(2)
dc.subjectMultidisciplinary Scienceses_CL
dc.titleThe fecal, oral, and skin microbiota of children with Chagas disease treated with benznidazolees_CL
dc.typeArtículoes_CL
umayor.facultadCIENCIAS
umayor.politicas.sherpa/romeoDOAJ Gold, Green Publishedes_CL
umayor.indexadoWOS:000459712100020es_CL
umayor.indexadoPMID: 30807605es_CL
dc.identifier.doiDOI: 10.1371/journal.pone.0212593es_CL]
umayor.indicadores.wos-(cuartil)Q2es_CL
umayor.indicadores.scopus-(scimago-sjr)SCIMAGO/ INDICE H: 268 Hes_CL


Vista simple de metadatos



Modificado por: Sistema de Bibliotecas Universidad Mayor - SIBUM
DSpace software copyright © 2002-2018  DuraSpace