Molecular signatures of extracellular vesicles in oral fluids of periodontitis patients
Fecha
2020Autor
Chaparro Padilla, Alejandra
Weber Aracena, Laura
Realini Fuentes, Ornella
Albers Busquetts, Daniela [Univ Mayor, Dept Stat, Fac Dent, Chile]
Hernández Ríos, Marcela
Ramírez Lobos, Valeria
La Rocca, Andrés Pascual
Nart Molina, José
Beltrán Varas, Víctor
Acuña Gallardo, Stephanie
Sanz Ruiz, Antonio
Ubicación geográfica
Notas
HERRAMIENTAS
Resumen
Objective To characterize extracellular vesicles (EVs) in gingival crevicular fluid (GCF) and saliva samples from healthy/gingivitis and periodontitis patients and correlate them with clinical inflammatory periodontal parameters. Material and Method An exploratory study, including 86 subjects, was conducted. Clinical and periodontal data were recorded, and oral fluid samples were obtained. EVs were precipitated by ExoQuick-TC (TM) and characterized by nanoparticle tracking (NanoSight (TM)), Western blot (WB), transmission electron microscopy (TEM), and ELISA analysis. Results TEM showed nanoparticles morphologically compatible with EVs, and WB analysis revealed bands of specific EV markers (CD9, TSG101, and Alix) in both oral fluids of periodontitis and healthy/gingivitis subjects. The total concentration of EVs in GCF was increased in periodontitis patients compared to healthy/gingivitis subjects (p = .017). However, we did not observe differences in the EV concentration of saliva samples (p = .190). The size of GCF-EVs was 144.2 nm in periodontitis and 160.35 nm in healthy/gingivitis patients (p = .038). The CD63 exosome marker was increased in GCF of periodontitis patients (p = .00001). The total concentration of EVs in GCF was correlated with bleeding on probing (rho = 0.63, p = .002), periodontal probing depth (rho = 0.56, p = .009), and clinical attachment level (rho = 0.48, p = .030). Conclusion Periodontitis patients have an increased concentration of EVs in GCF, and their role in periodontitis should be clarified.
URI
http://repositorio.umayor.cl/xmlui/handle/sibum/8019https://doi.org/10.1111/odi.13338
http://repositorio.uchile.cl/handle/2250/174929
https://onlinelibrary.wiley.com/doi/10.1111/odi.13338
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