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dc.contributorBIOMED CENTRAL LTDes
dc.contributor.authorSepúlveda Cisternas, Ignacio [Chile. Universidad Mayor. Facultad de Ciencias. Escuela de Biotecnología]
dc.contributor.authorTorres, Alexia [Chile. Universidad de Chile. Facultad de Medicina. Instituto de Ciencias Biomédicas. Programa de Microbiología y Micología]
dc.contributor.authorFuentes Flores, Andrés [Chile. Universidad de Chile. Facultad de Medicina. Instituto de Ciencias Biomédicas. Programa de Microbiología y Micología]
dc.contributor.authorGarcía Angulo, Víctor Antonio [Chile. Universidad de Chile. Facultad de Medicina. Instituto de Ciencias Biomédicas. Programa de Microbiología y Micología]
dc.date.accessioned2018-05-10T21:53:13Z
dc.date.available2018-05-10T21:53:13Z
dc.date.issued2017-02-15
dc.identifier.citationCisternas I, Torres A, Flores A, García Angulo V. Differential regulation of riboflavin supply genes in Vibrio cholerae. Gut Pathogens [serial on the Internet]. (2017, Feb 15), [cited May 10, 2018]; 91-7es
dc.identifier.issn1757-4749
dc.identifier.otherWOS:000394812700001
dc.identifier.otherID de PubMed: 28239422
dc.identifier.urihttp://repositorio.umayor.cl/xmlui/handle/sibum/437
dc.identifier.urihttps://doi.org/10.1186/s13099-017-0159-z
dc.description.abstractBackground: Riboflavin is the precursor of important redox cofactors such as flavin mononucleotide (FMN) and flavin adenine dinucleotide, required for several biological processes. Vibrio cholerae, a pathogenic bacterium responsible for the cholera disease, possesses the ability to biosynthesize de novo as well as to uptake riboflavin through the riboflavin biosynthetic pathway (RBP) and the RibN importer, respectively. The intra-organism relationship between riboflavin biosynthesis and uptake functions has not been studied. Results: This work determined the transcriptional organization of RBP genes and ribN in V. cholerae through reverse transcription polymerase chain reaction and analyzed their expression when growing with or without extracellular riboflavin using real time PCR. The RBP is organized in three transcriptional units, the major one containing ribD, ribE, ribA and ribH together with genes involved in functions not directly related to riboflavin biosynthesis such as nrdR and nusB. In addition, two independent monocistronic units contain ribA2 and ribB, the later conserving a putative FMN riboswitch. The ribN gene is encoded in operon with a gene coding for a predicted outer membrane protein and a gene encoding a protein with a glutaredoxin domain. Regulation analysis showed that among these transcriptional units, only ribB is negatively regulated by riboflavin and that its repression depends on the RibN riboflavin importer. Moreover, external riboflavin highly induced ribB transcription in a ribN strain. Also, a genomic database search found a negative correlation between the presence of nrdR and nusB and the FMN riboswitch in bacterial RBP operons. Conclusions: Growing in the presence of riboflavin downregulates only a single element among the transcriptional units of riboflavin supply pathways. Thus, endogenous riboflavin biosynthesis seems to be negatively regulated by extracellular riboflavin through its specific effect on transcription of ribB in V. choleraees
dc.description.sponsorshipEste trabajo fue financiado por el proyecto FONDECYT Regular (CONICYT Chile), Grant 1150818.es
dc.format.extentPDFes
dc.language.isoenes
dc.publisherChile. Universidad Mayores
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chilees
dc.subject.meshVibrio choleraees
dc.subject.meshRiboflavina/biosíntesises
dc.subject.meshRiboflavina/metabolismoes
dc.subject.meshIntestinos/microbiologíaes
dc.titleDifferential regulation of riboflavin supply genes in Vibrio choleraees
dc.title.alternativeRegulación diferencial de los genes de suministro de riboflavina en Vibrio choleraees
dc.typeArtículo o Paperes
umayor.indizadorCOTes
umayor.politicas.sherpa/romeohttp://www.sherpa.ac.uk/romeo/issn/1757-4749/es
umayor.indexadoWeb of Sciencees
dc.identifier.doi10.1186/s13099-017-0159-z
umayor.indicadores.wos-(cuartil)Q3


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