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dc.contributor.authorHolmes, David S. [Univ Mayor, Fac Ciencias, Ctr Genom & Bioinformat, Santiago, Chile]es_CL
dc.contributor.authorOsorio, Hectores_CL
dc.contributor.authorMettert, Erines_CL
dc.contributor.authorKiley, Patriciaes_CL
dc.contributor.authorDopson, Markes_CL
dc.contributor.authorJedlicki, Eugeniaes_CL
dc.date.accessioned2020-04-12T14:11:55Z
dc.date.accessioned2020-04-14T15:37:59Z
dc.date.available2020-04-12T14:11:55Z
dc.date.available2020-04-14T15:37:59Z
dc.date.issued2019es_CL
dc.identifier.citationOsorio, H., Mettert, E. L., Kiley, P., Dopson, M., Jedlicki, E., & Holmes, D. S. (2019). Identification and Unusual Properties of the Master Regulator FNR in the Extreme Acidophile Acidithiobacillus ferrooxidans. Frontiers in microbiology, 10, 1642.es_CL
dc.identifier.issn1664-302Xes_CL
dc.identifier.urihttps://doi.org/10.3389/fmicb.2019.01642es_CL
dc.identifier.urihttp://repositorio.umayor.cl/xmlui/handle/sibum/6612
dc.description.abstractThe ability to conserve energy in the presence or absence of oxygen provides a metabolic versatility that confers an advantage in natural ecosystems. The switch between alternative electron transport systems is controlled by the fumarate nitrate reduction transcription factor (FNR) that senses oxygen via an oxygen-sensitive [4Fe-4S](2+) iron-sulfur cluster. Under O-2 limiting conditions, FNR plays a key role in allowing bacteria to transition from aerobic to anaerobic lifestyles. This is thought to occur via transcriptional activation of genes involved in anaerobic respiratory pathways and by repression of genes involved in aerobic energy production. The Proteobacterium Acidithiobacillus ferrooxidans is a model species for extremely acidophilic microorganisms that are capable of aerobic and anaerobic growth on elemental sulfur coupled to oxygen and ferric iron reduction, respectively. In this study, an FNR-like protein (FNRAF) was discovered in At. ferrooxidans that exhibits a primary amino acid sequence and major motifs and domains characteristic of the FNR family of proteins, including an effector binding domain with at least three of the four cysteines known to coordinate an [4Fe-4S](2+) center, a dimerization domain, and a DNA binding domain. Western blotting with antibodies against Escherichia coli FNR (FNREC) recognized FNRAF. FNRAF was able to drive expression from the FNR-responsive E. coli promoter PnarG, suggesting that it is functionally active as an FNR-like protein. Upon air exposure, FNRAF demonstrated an unusual lack of sensitivity to oxygen compared to the archetypal FNREC. Comparison of the primary amino acid sequence of FNRAF with that of other natural and mutated FNRs, including FNREC, coupled with an analysis of the predicted tertiary structure of FNRAF using the crystal structure of the related FNR from Aliivibrio fisheri as a template revealed a number of amino acid changes that could potentially stabilize FNRAF in the presence of oxygen. These include a truncated N terminus and amino acid changes both around the putative Fe-S cluster coordinating cysteines and also in the dimer interface. Increased O-2 stability could allow At. ferrooxidans to survive in environments with fluctuating O-2 concentrations, providing an evolutionary advantage in natural, and engineered environments where oxygen gradients shape the bacterial community.es_CL
dc.description.sponsorshipPrograma de Apoyo a Centros con Financiamiento Basal [AFB 17004]; FondecytComision Nacional de Investigacion Cientifica y Tecnologica (CONICYT)CONICYT FONDECYT [1181717]; NIHUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USA [R01-GM115894, R01-GM045844]es_CL
dc.description.sponsorshipThis work was supported by the Programa de Apoyo a Centros con Financiamiento Basal AFB 17004 to Fundacion Ciencia & Vida and Fondecyt 1181717 to DH. The work in the Kiley lab was supported by the NIH grants R01-GM115894 and R01-GM045844 to PK.es_CL
dc.language.isoenes_CL
dc.publisherFRONTIERS MEDIA SAes_CL
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile
dc.sourceFront. Microbiol., JUL, 2019. 10
dc.subjectMicrobiologyes_CL
dc.titleIdentification and Unusual Properties of the Master Regulator FNR in the Extreme Acidophile Acidithiobacillus ferrooxidanses_CL
dc.typeArtículoes_CL
umayor.facultadCIENCIAS
umayor.politicas.sherpa/romeoDOAJ Gold, Green Publishedes_CL
umayor.indexadoWOS:000476728500001es_CL
umayor.indexadoPMID: 31379789es_CL
dc.identifier.doiDOI: 10.3389/fmicb.2019.01642es_CL]
umayor.indicadores.wos-(cuartil)Q1es_CL
umayor.indicadores.scopus-(scimago-sjr)SCIMAGO/ INDICE H: 88 Hes_CL


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