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dc.contributor.authorReyes-Cerpa, Sebastián [Univ Mayor, Ctr Genom & Bioinformat, Fac Ciencias, Santiago, Chile]es_CL
dc.contributor.authorValdés, Jorge [Univ Mayor, Ctr Genom & Bioinformat, Fac Ciencias, Santiago, Chile]es_CL
dc.contributor.authorIbaceta, Valentina [Univ Mayor, Ctr Genom & Bioinformat, Fac Ciencias, Santiago, Chile]es_CL
dc.contributor.authorBrianson, Bernardo [Univ Mayor, Ctr Genom & Bioinformat, Fac Ciencias, Santiago, Chile]es_CL
dc.contributor.authorEspinoza, Allison [Univ Mayor, Ctr Genom & Bioinformat, Fac Ciencias, Santiago, Chile]es_CL
dc.contributor.authorAhumada, Diego E. [Univ Mayor, Ctr Genom & Bioinformat, Fac Ciencias, Santiago, Chile]es_CL
dc.contributor.authorTapia, Sebastián [Univ Mayor, Ctr Genom & Bioinformat, Fac Ciencias, Santiago, Chile]es_CL
dc.contributor.authorPérez-Stuardo, Diego [Univ Mayor, Ctr Genom & Bioinformat, Fac Ciencias, Santiago, Chile]es_CL
dc.contributor.authorMorales-Reyes, Jonathan; Espinoza, Allison; Soto-Herrera, Valentina; Sandino, Ana M.; Spencer, Eugenio; Vallejos-Vidal, Eva; Reyes-López, Felipe E.es_CL
dc.date.accessioned2020-04-12T14:11:55Z
dc.date.accessioned2020-04-14T15:46:15Z
dc.date.available2020-04-12T14:11:55Z
dc.date.available2020-04-14T15:46:15Z
dc.date.issued2019es_CL
dc.identifier.citationPérez-Stuardo, D., Morales-Reyes, J., Tapia, S., Ahumada, D. E., Espinoza, A., Soto-Herrera, V., ... & Vallejos-Vidal, E. (2019). Non-lysosomal activation in macrophages of Atlantic salmon (Salmo salar) after infection with Piscirickettsia salmonis. Frontiers in immunology, 10, 434.es_CL
dc.identifier.issn1664-3224es_CL
dc.identifier.urihttps://doi.org/10.3389/fimmu.2019.00434es_CL
dc.identifier.urihttp://repositorio.umayor.cl/xmlui/handle/sibum/6684
dc.description.abstractPiscirickettsia salmonis is a facultative intracellular pathogen and etiological agent of the systemic disease salmonid rickettsial septicemia. It has been suggested that P salmonis is able to survive in host macrophages, localized within a vacuole like-compartment which prevents lysosomal degradation. However, the relevant aspects of the pathogenesis of P salmonis as the host modulation that allow its intracellular survival have been poorly characterized. In this study, we evaluated the role of lysosomes in the response to P salmonis infection in macrophage-enriched cell cultures established from Atlantic salmon head kidneys. Bacterial infection was confirmed using confocal microscopy. A gentamicin protection assay was performed to recover intracellular bacteria and the 16S rDNA copy number was quantified through quantitative polymerase chain reaction in order to determine the replication of P salmonis within macrophages. Lysosomal activity in Atlantic salmon macrophage-enriched cell cultures infected with P salmonis was evaluated by analyzing the lysosomal pH and proteolytic ability through confocal microscopy. The results showed that P salmonis can survive >= 120 h in Atlantic salmon macrophage-enriched cell cultures, accompanied by an increase in the detection of the 16S rDNA copy number/cell. The latter finding suggests that P salmonis also replicates in Atlantic salmon macrophage-enriched cell cultures. Moreover, this bacterial survival and replication appears to be favored by a perturbation of the lysosomal degradation system. We observed a modulation in the total number of lysosomes and lysosomal acidification following infection with P salmonis. Collectively, the results of this study showed that infection of Atlantic salmon macrophages with P salmonis induced limited lysosomal response which may be associated with host immune evasion mechanisms of P salmonis that have not been previously reported.es_CL
dc.description.sponsorshipFONDECYTComision Nacional de Investigacion Cientifica y Tecnologica (CONICYT)CONICYT FONDECYT [11150807]; Start-Up UMayor fellowship [101205]; CORFO fellowship [13CTI-21527]; UMayor-Ph.D. fellowship; CONICYT-BCH Postdoctoral fellowshipes_CL
dc.description.sponsorshipThis work was financially supported by FONDECYT Iniciacion 11150807, Start-Up UMayor 101205, CORFO 13CTI-21527, UMayor-Ph.D. fellowships to DP-S and CONICYT-BCH Postdoctoral fellowship to EV-V.es_CL
dc.language.isoenes_CL
dc.publisherFRONTIERS MEDIA SAes_CL
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile
dc.sourceFront. Immunol., MAR, 2019. 10
dc.subjectImmunologyes_CL
dc.titleNon-lysosomal Activation in Macrophages of Atlantic Salmon (Salmo salar) After Infection With Piscirickettsia salmonises_CL
dc.typeArtículoes_CL
umayor.facultadCIENCIAS
umayor.politicas.sherpa/romeoDOAJ Gold, Green Publishedes_CL
umayor.indexadoWOS:000461590500001es_CL
umayor.indexadoPMID: 30941123es_CL
dc.identifier.doiDOI: 10.3389/fimmu.2019.00434es_CL]
umayor.indicadores.wos-(cuartil)Q2es_CL
umayor.indicadores.scopus-(scimago-sjr)SCIMAGO/ INDICE H: 84 Hes_CL


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